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KMID : 0848120190440030096
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International Journal of Oral Biology
2019 Volume.44 No. 3 p.96 ~ p.100
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Peptoniphilus mikwangii -specific quantitative real-time polymerase chain reaction primer
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Park Soon-Nang
Kook Joong-Ki
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Abstract
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The purpose of this study was to develop Peptoniphilus mikwangii -specific quantitative real-time polymerase chain reaction (qPCR) primers based on the 16S ribosomal RNA (16S rDNA) gene. The specificity of the primers was determined by conventional PCR using 29 strains of 27 oral bacterial species including P. mikwangii . The sensitivity of the primers was determined by qPCR using the purified genomic DNA of P. mikwangii KCOM 1628 T (40 ng to 4 fg). The data showed that the qPCR primers (RTB134-F4/RTB134-R4) could detect P. mikwangii strains exclusively and as little as 40 fg of the genomic DNA of P. mikwangii KCOM 1628 T . These results suggest that the developed qPCR primer pair can be useful for detecting P. mikwangii in epidemiological studies of oral bacterial infectious diseases.
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KEYWORD
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Peptoniphilus mikwangii, 16S rDNA, qPCR primers
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